Lukiw W J, Cui J G, Li Y Y, Culicchia F
LSU Neuroscience Center of Excellence and LSU Department of Neurosurgery, Louisiana State University Health Sciences Center, 2020 Gravier Street, Suite 8A4, New Orleans, LA, 70112, USA.
J Neurooncol. 2009 Jan;91(1):27-32. doi: 10.1007/s11060-008-9688-0. Epub 2008 Aug 31.
Glioblastoma multiforme (GBM) represents a class of malignant gliomas which rapidly proliferate, invade and destroy surrounding brain tissues. This study examined micro-RNA (miRNA) speciation and miRNA effects on gene expression in six ATCC glioma and GBM cell lines and in 14 glioma and GBM samples obtained from human brain biopsy. We observed selective up-regulation of miRNA-221 and down-regulation of a miRNA-221 messenger RNA target encoding the survivin-1 homolog BIRC1, a neuronal inhibitor of apoptosis protein (NIAP) and marker for neurodegeneration. The expression of BIRC5 (survivin-1) and caspase-3 were found to be significantly up-regulated, particularly in stage IV GBM. These studies suggest that the abundance and speciation of the BIRC family of neural cell fate regulators are differentially regulated in glioma and GBM, and may contribute to progressive changes in apoptotic signaling and altered neural cell cycling functions.
多形性胶质母细胞瘤（GBM）是一类恶性胶质瘤，其能迅速增殖、侵袭并破坏周围脑组织。本研究检测了六种美国典型培养物保藏中心（ATCC）的胶质瘤和GBM细胞系以及14例取自人脑活检的胶质瘤和GBM样本中的微小RNA（miRNA）种类及其对基因表达的影响。我们观察到miRNA - 221选择性上调，而编码survivin - 1同源物BIRC1（一种神经元凋亡抑制蛋白（NIAP）和神经退行性变标志物）的miRNA - 221信使核糖核酸靶标下调。发现BIRC5（survivin - 1）和半胱天冬酶 - 3的表达显著上调，尤其在IV期GBM中。这些研究表明，神经细胞命运调节因子BIRC家族的丰度和种类在胶质瘤和GBM中受到不同调控，可能导致凋亡信号的渐进性变化以及神经细胞周期功能改变。
