人类扁桃体癌中蛋白质表达的蛋白质组学分析：差异表达蛋白可表征人类扁桃体癌。

Proteomic analysis of protein expression in human tonsillar cancer: differentially expressed proteins characterize human tonsillar cancer.

作者信息

Roblick Uwe J, Bader Franz G, Hammarstedt Lalle, Habermann Jens K, Hellman Ulf, Becker Susanne, Sundmacker Axel, Gemoll Timo, Zimmermann Kaja, Auer Gert, Munck-Wikland Eva

机构信息

Department of Surgery and Laboratory for Surgical Research, University of Schleswig-Holstein, Luebeck, Germany.

出版信息

Acta Oncol. 2008;47(8):1493-501. doi: 10.1080/02841860802314696.

DOI:10.1080/02841860802314696

PMID:18759147

Abstract

BACKGROUND

Head and neck cancer continues to be one of the most common tumor entities worldwide. Within this group of malignancies, tonsillar squamous cell carcinoma represent approximately 15-20% of all intraoral and oropharyngeal carcinomas in the United States. Accurate and early stage diagnosis still remains a major challenge, as patients are often presented at an advanced stage of disease, causing a low overall survival rate. Thus, new diagnostic markers are highly desirable and could allow for a more reliable diagnosis, with further insights into carcinogenesis and tumor biology. Furthermore, these markers could be the basis for new therapeutic targets and early disease detection. To address these issues, we decided to use a global proteomic approach to characterize tonsillar squamous cell carcinoma.

MATERIALS AND METHODS

A total of 19 tonsillar carcinoma samples and 12 benign controls acquired from the corresponding normal epithelium were analyzed by 2-D gel electrophoresis. 2-DE gels were silver stained and analyzed using the PDQuest analysis software (BioRad). Tumor specific spots were detected and identified by consecutive MALDI-TOF-MS or MS/MS polypeptide identification.

RESULTS

In total, 70 proteins showed significant quantitative differences in protein expression, with 50 polypeptides accessible for identification. Of those 50 polypeptides, we were able to identify a total of 27 proteins and protein isoforms, significantly up- or down-regulated in tonsillar cancer samples. In addition to previously reported polypeptides in head and neck cancers, we were able to identify several new potential marker proteins in this study.

CONCLUSION

Our results show that a combination of tonsillar cancer specific proteins can be used for histopathological diagnosis and may serve as a basis for discovering further biomarkers for early detection and prediction of response to treatment in the future.

摘要

背景

头颈癌仍然是全球最常见的肿瘤类型之一。在这组恶性肿瘤中，扁桃体鳞状细胞癌约占美国所有口腔和口咽癌的15 - 20%。准确的早期诊断仍然是一项重大挑战，因为患者常常在疾病晚期才就诊，导致总体生存率较低。因此，非常需要新的诊断标志物，这有助于实现更可靠的诊断，并能进一步深入了解肿瘤发生和肿瘤生物学。此外，这些标志物可以成为新的治疗靶点和早期疾病检测的基础。为了解决这些问题，我们决定采用全球蛋白质组学方法来表征扁桃体鳞状细胞癌。