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连接探针——通过模板增强杂交过程对核酸进行序列特异性检测。

Junction probes - sequence specific detection of nucleic acids via template enhanced hybridization processes.

作者信息

Nakayama Shizuka, Yan Lei, Sintim Herman O

机构信息

Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.

出版信息

J Am Chem Soc. 2008 Sep 24;130(38):12560-1. doi: 10.1021/ja803146f. Epub 2008 Aug 29.

Abstract

Junction probe nucleic acid detection technology allows the amplified sensing of analytes at isothermal conditions. The addition of a second dimension to detection probes permits the use of cheap commercially available DNA processing enzymes such as restriction endonucleases to detect single nucleotide polymorphisms, SNPs.

摘要

连接探针核酸检测技术可在等温条件下对分析物进行扩增检测。在检测探针中增加第二个维度,使得能够使用诸如限制性内切酶等廉价的市售DNA处理酶来检测单核苷酸多态性(SNP)。

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