Islas-Rodríguez A E, Guillen-Vargas C, Villa-Manzanarez L, González-Mendoza A
Instituto de Patología Infecciosa y Experimental, Universidad de Guadalajara, Hospital 308, Jalisco, México.
Mycopathologia. 1991 May;114(2):77-81. doi: 10.1007/BF00436425.
The incorporation of (3H) thymidine and the biosynthesis of interleukin-2(IL-2) were investigated in Concanavalin A (ConA) and histoplasmin stimulated lymphocytes from spleen of infected Balb/c mice with the yeast phases of Histoplasma capsulatum. The ability to incorporate (3H) thymidine of Con A stimulated lymphocytes in culture from spleen of Histoplasma capsulatum infected mice, as well as the IL-2 content present in the supernatants of that cultures, were depressed along the first three weeks of the experiments, but starting week five, normal values were restored or even discretly increased. Incorporation of (3H) thymidine in histoplasmin stimulated lymphocytes remained inhibited along the seven weeks the experiment lasted. Results showed that inoculation of H. capsulatum yeast in mice provoked a temporary immunosuppression on cell mediated immunity, that can be explained by means of the inability of T cells to produce enough IL-2 necessary for the proliferation of T cells in culture.
在感染荚膜组织胞浆菌酵母相的Balb/c小鼠脾脏中，研究了伴刀豆球蛋白A（ConA）和组织胞浆菌素刺激的淋巴细胞中（3H）胸腺嘧啶核苷的掺入及白细胞介素-2（IL-2）的生物合成。在实验的前三周，荚膜组织胞浆菌感染小鼠脾脏中ConA刺激的淋巴细胞掺入（3H）胸腺嘧啶核苷的能力以及该培养物上清液中存在的IL-2含量均降低，但从第五周开始，恢复到正常水平，甚至略有增加。在整个实验持续的七周内，组织胞浆菌素刺激的淋巴细胞中（3H）胸腺嘧啶核苷的掺入仍受到抑制。结果表明，给小鼠接种荚膜组织胞浆菌酵母会引起细胞介导免疫的暂时免疫抑制，这可以通过T细胞无法产生培养中T细胞增殖所需的足够IL-2来解释。
