Gidwitz S, Weber M J, Storm D R
J Biol Chem. 1976 Dec 25;251(24):7950-1.
Adenylate cyclase activities in membranes prepared from Rous sarcoma-transformed chicken embryo fibroblasts are 2 to 4 times lower than in membranes prepared from normal chicken embryo fibroblasts. Adenylate cyclase activities were solubilized from normal and transformed membranes with five different nonionic detergents. In all cases, the specific activities of the enzyme solubilized from normal and transformed preparations were essentially identical. These data suggest that the microenvironment of adenylate cyclase in transformed membranes may be wholly or partially responsible for the decreased activities of this enzyme.
从劳斯肉瘤转化的鸡胚成纤维细胞制备的膜中的腺苷酸环化酶活性比从正常鸡胚成纤维细胞制备的膜中的活性低2至4倍。用五种不同的非离子去污剂从正常和转化的膜中溶解腺苷酸环化酶活性。在所有情况下，从正常和转化制剂中溶解的酶的比活性基本相同。这些数据表明，转化膜中腺苷酸环化酶的微环境可能全部或部分导致该酶活性降低。
