Liu Dali, Yumoto Hiromichi, Murakami Keiji, Hirota Katsuhiko, Kayama Shizuo, Taniguchi Tomonori, Yamamoto Akitake, Ono Tsuneko, Matsuo Takashi, Miyake Yoichiro
FEMS Microbiol Lett. 2008 Nov;288(1):68-75. doi: 10.1111/j.1574-6968.2008.01327.x.
Escherichia coli failed to survive after transformation with a Streptococcus intermedius histone-like protein gene (Si-hlp) and its promoter-harbored plasmid. The promoter function of Si-hlp in E. coli was determined using enhanced green fluorescence protein (egfp) gene as a reporter. The inhibitory effect of Si-HLP on E. coli viability was verified by a tetracycline-inducible gene expression system. Further study suggested that Si-HLP may alter the bacterial nucleoid structure, leading to the growth inhibition of E. coli.
用中间型链球菌组蛋白样蛋白基因（Si-hlp）及其携带启动子的质粒转化大肠杆菌后，大肠杆菌无法存活。以增强型绿色荧光蛋白（egfp）基因作为报告基因，测定了Si-hlp在大肠杆菌中的启动子功能。通过四环素诱导基因表达系统验证了Si-HLP对大肠杆菌活力的抑制作用。进一步研究表明，Si-HLP可能改变细菌类核结构，导致大肠杆菌生长受到抑制。
