Gómez-Eichelmann M C, Camacho-Carranza R
Departamento de Biología Molecular, Universidad Nacional Autónoma de México.
Rev Latinoam Microbiol. 1995 Jul-Sep;37(3):281-90.
The bacterial genome is present in the cell within a complex structure, the nucleoid. The nucleoid contains the genomic DNA, and molecules of RNA and proteins. The main proteins of the nucleoid are: RNA polymerase, topoisomerases and the histone-like proteins: HU, H-NS (H1), H, HLP1, IHF and FIS. The DNA molecule in the nucleoid is under helical tension or supercoiling and is organized into 43 +/- 10 topodomains. DNA supercoiling is generated by the activity of the topoisomerases and by DNA-protein interactions. In this review, we analize current knowledge in Escherichia coli about genome organization and proteins of the nucleoid.
细菌基因组以一种复杂的结构——类核存在于细胞中。类核包含基因组DNA、RNA分子和蛋白质分子。类核的主要蛋白质有：RNA聚合酶、拓扑异构酶以及类组蛋白：HU、H-NS（H1）、H、HLP1、IHF和FIS。类核中的DNA分子处于螺旋张力或超螺旋状态，并被组织成43 +/- 10个拓扑结构域。DNA超螺旋是由拓扑异构酶的活性以及DNA-蛋白质相互作用产生的。在这篇综述中，我们分析了大肠杆菌中关于基因组组织和类核蛋白质的现有知识。
