生物分子核磁共振实验中用于水峰抑制的相移预饱和。
Phase-shift presaturation for water peak suppression in biomolecular NMR experiments.
作者信息
Mao X, Ye C
机构信息
Chinese Academy of Sciences, Wuhan, China.
出版信息
Sci China C Life Sci. 1997 Aug;40(4):345-50. doi: 10.1007/BF02881726.
PMID:18762872
Abstract
The phase-shift presaturation technique for water suppression is further improved with effective phase cycling. In the two-dimensional NMR COSY, NOESY and HMQC experiments of biosamples, the water suppression factor reaches the order of 10(-6), making it possible to detect(13)C NMR signals in extremely diluted (2 mmol/L) aqueous solutions.
摘要
通过有效的相位循环进一步改进了用于水抑制的相移预饱和技术。在生物样品的二维核磁共振COSY、NOESY和HMQC实验中，水抑制因子达到10^(-6)量级，使得在极稀（2 mmol/L）水溶液中检测^(13)C NMR信号成为可能。
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