Andreeva L E, Khaĭdarova N V, Sleptsova L A, Rodriges-Blanko E V, Dicheva M A, Dvorianchikov G A, Tarantul V Z
Genetika. 2008 Jul;44(7):992-9.
Transient expression of recombinant plasmids carrying the lacZ gene under the control of either bovine alphaS1-casein gene tissue-specific promoter-enhancer region or highly homologous goat alphaS1-casein gene promoter-enhancer region with supplementary regulatory sequences of the goat gene were studied in Misgurnus fossilis L. loach embryos. It has been shown previously that the expression of the constructs carrying these regulatory elements in transgenic mice occurred primarily in the mammary glands. At early developmental stages, loach embryos and early prelarvae showed nonspecific and mosaic transient expression of lacZ carrying casein regulatory sequences. Transgenic activity was the highest in 1-3-day embryos. At the same time, the efficiency of expression of lacZ gene carrying regulatory sequences of the alphaS1-casein gene of goat was higher than with the promoter-enhancer region of the bovine alphaS1-casein gene. Thus, regulatory sequences of the bovine or goat alphaS1-casein gene appeared capable of providing similar transient expression of reporter gene in the loach embryos. This model can be used for rapid testing of promoter-enhancer activity of transgenes.
在泥鳅胚胎中研究了携带lacZ基因的重组质粒的瞬时表达，这些重组质粒受牛αS1-酪蛋白基因组织特异性启动子-增强子区域或高度同源的山羊αS1-酪蛋白基因启动子-增强子区域以及山羊基因的补充调控序列的控制。先前已表明，携带这些调控元件的构建体在转基因小鼠中的表达主要发生在乳腺中。在发育早期阶段，泥鳅胚胎和早期仔鱼表现出携带酪蛋白调控序列的lacZ的非特异性和镶嵌性瞬时表达。转基因活性在1-3日龄胚胎中最高。同时，携带山羊αS1-酪蛋白基因调控序列的lacZ基因的表达效率高于牛αS1-酪蛋白基因的启动子-增强子区域。因此，牛或山羊αS1-酪蛋白基因的调控序列似乎能够在泥鳅胚胎中提供相似的报告基因瞬时表达。该模型可用于快速测试转基因的启动子-增强子活性。
