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呼吸细胞色素掺入脂质体:研究呼吸链的有效策略。

Incorporation of respiratory cytochromes in liposomes: an efficient strategy to study the respiratory chain.

作者信息

Nantes Iseli Lourenco, Mugnol Katia Cristina Ugolini

机构信息

Centro Interdisciplinar de Investigação Bioquimica-CIIB, Universidade de Mogi das Cruzes-UMC, Mogi das Cruzes-SP, Brazil.

出版信息

J Liposome Res. 2008;18(3):175-94. doi: 10.1080/08982100802340367.

Abstract

The electron transport system present in the photosynthetic apparatus and respiratory chain were evolutionary acquisitions that allowed the organisms to convert electromagnetic energy in chemical energy and thus improve the use of energy fuels. These systems acquired by prokaryotes were preserved in the highly complex organisms always with the participation of the cytochromes. Since the discovery of the cytochromes, and the isolation and association of these proteins to model membranes, the liposomes, have been used to investigative strategies to characterize the structure and function of these proteins. From these studies important findings have contributed to the comprehension of the energy transduction mechanisms and the role played by the nonredox subunits present in the protein complexes of the respiratory chain of eukaryotes.

摘要

光合装置和呼吸链中存在的电子传递系统是进化过程中获得的,使生物体能够将电磁能转化为化学能,从而提高能量燃料的利用效率。原核生物获得的这些系统在高度复杂的生物体中得以保留,细胞色素始终参与其中。自从发现细胞色素,并将这些蛋白质分离并与模型膜(脂质体)结合以来,脂质体已被用于研究策略,以表征这些蛋白质的结构和功能。从这些研究中,重要的发现有助于理解能量转导机制以及真核生物呼吸链蛋白质复合物中存在的非氧化还原亚基所起的作用。

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Study of respiratory cytochromes in liposomes.脂质体中呼吸细胞色素的研究。
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