Koyama H, Hodatsu T, Sasaki T, Ohwada Y, Saito Y, Saito H
Department of Microbiology, School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori, Japan.
Avian Pathol. 1981 Apr;10(2):151-62. doi: 10.1080/03079458108418469.
Oncogenic reticuloendotheliosis virus strain T (REV-T) was inoculated into 9-day-old embryonated eggs of specific pathogen free chickens. A continuous cell line was established from the liver cells of one embryo harvested the day before hatching which had the following properties: It formed loose aggregates and propagated floating in the medium. The cell doubling time was about 24 hours. Viral antigen was demonstrated in the cytoplasm by the direct immunofluorescent antibody technique. C-type particles were observed outside the cells. Virus-induced cell surface antigen was demonstrated by the immunofluorescent antibody technique. The cell line grew in soft agar. Virus production continued in the cell line for 17 months. When the cells or the culture fluid were inoculated into 1-day-old chickens, they died from neoplastic disease. The chickens which survived after infection showed abnormal feathering ("nakanuke"). The virus released from this cell-line was inoculated into 9-day-old embryonated eggs and three cell lines were established. These cell lines did not contain avian leukosis/sarcoma viruses.
将致癌性网状内皮增生症病毒T株（REV-T）接种到无特定病原体鸡的9日龄胚胎蛋中。从孵化前一天收获的一个胚胎的肝细胞建立了一个连续细胞系，该细胞系具有以下特性：它形成松散聚集体并在培养基中漂浮繁殖。细胞倍增时间约为24小时。通过直接免疫荧光抗体技术在细胞质中检测到病毒抗原。在细胞外观察到C型颗粒。通过免疫荧光抗体技术检测到病毒诱导的细胞表面抗原。该细胞系在软琼脂中生长。病毒在该细胞系中持续产生了17个月。当将细胞或培养液接种到1日龄鸡中时，它们死于肿瘤性疾病。感染后存活的鸡表现出羽毛异常（“中拔”）。从该细胞系释放的病毒接种到9日龄胚胎蛋中，并建立了三个细胞系。这些细胞系不含有禽白血病/肉瘤病毒。
