Taga Michiko E
Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Curr Protoc Microbiol. 2005 Jul;Chapter 1:Unit 1C.1. doi: 10.1002/9780471729259.mc01c01s00.
The quorum-sensing signal molecule autoinducer-2 (AI-2) is produced by over 50 diverse bacterial species and controls many different processes, including antibiotic production, biofilm formation, and virulence. AI-2 production often varies according to growth phase, media conditions, and the presence of specific factors. This unit describes a biological assay for AI-2 activity produced by a bacterial strain of interest. The assay employs an AI-2 reporter strain, Vibrio harveyi BB170, which produces light in response to AI-2. In the first stage of the assay, culture fluids of the bacterial strain of interest are collected over a time course of growth and filtered to remove cells. In the next stage, these culture fluids are mixed with BB170, and the light produced in response to AI-2 in the culture fluids is measured using a luminometer. BB170 is exquisitely sensitive to AI-2, and therefore, even low amounts of AI-2 can be detected using this bioassay.
群体感应信号分子自诱导物-2（AI-2）由50多种不同细菌产生，并控制许多不同过程，包括抗生素生产、生物膜形成和毒力。AI-2的产生通常会根据生长阶段、培养基条件和特定因素的存在而有所不同。本单元描述了一种针对感兴趣的细菌菌株产生的AI-2活性的生物学检测方法。该检测方法使用AI-2报告菌株哈维氏弧菌BB170，它会对AI-2产生反应而发光。在检测的第一阶段，在感兴趣的细菌菌株生长的时间过程中收集培养液，并过滤以去除细胞。在下一阶段，将这些培养液与BB170混合，并使用光度计测量培养液中因AI-2产生的光。BB170对AI-2极其敏感，因此，使用这种生物检测方法甚至可以检测到少量的AI-2。
