Villamor Neus
Hospital Clínic de Barcelona, Barcelona, Spain.
Curr Protoc Cytom. 2005 May;Chapter 6:Unit 6.19. doi: 10.1002/0471142956.cy0619s32.
Chronic lymphocytic leukemia (CLL) is the most common chronic leukemia in Western countries. The disease has an extremely variable clinical course, and several prognostic features have been identified to assess individual risk. The configuration of the immunoglobulin variable heavy-chain gene (IgV(H)) is a strong predictor of the outcome. CLL patients with unmutated IgV(H) status have an aggressive clinical course and a short survival. Unfortunately, analysis of IgV(H) gene configuration is not available in most clinical laboratories. A small number of genes are differentially expressed between unmutated IgV(H) and mutated IgV(H) clinical forms of CLL. One of these genes is ZAP-70, which is detected in leukemic cells from patients with the unmutated IgV(H) form of CLL. Flow cytometry presents advantages over other methods to detect ZAP-70, and its quantification by flow cytometry has proved its predictive value. This unit focuses on protocols to quantify ZAP-70 by flow cytometry in CLL.
慢性淋巴细胞白血病（CLL）是西方国家最常见的慢性白血病。该疾病的临床病程极为多变，已确定了多种预后特征以评估个体风险。免疫球蛋白可变重链基因（IgV(H)）的构型是预后的有力预测指标。IgV(H) 未突变的CLL患者临床病程侵袭性强，生存期短。遗憾的是，大多数临床实验室无法进行IgV(H) 基因构型分析。在CLL的IgV(H) 未突变和突变临床形式之间，少数基因存在差异表达。其中一个基因是ZAP-70，在IgV(H) 未突变形式CLL患者的白血病细胞中可检测到。流式细胞术在检测ZAP-70方面比其他方法具有优势，并且通过流式细胞术对其进行定量已证明了其预测价值。本单元重点介绍通过流式细胞术对CLL中的ZAP-70进行定量的方案。
