研究血管生成相关信号转导的方法。

Approaches for studying angiogenesis-related signal transduction.

作者信息

Derakhshan Behrad, Harrison Kenneth D, Miao Qing Robert, Sessa William C

机构信息

Vascular Biology and Therapeutics Program, Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut, USA.

出版信息

Methods Enzymol. 2008;443:1-23. doi: 10.1016/S0076-6879(08)02001-6.

DOI:10.1016/S0076-6879(08)02001-6

PMID:18772008

Abstract

Understanding how extracellular growth factors activate intracellular pathways that promote angiogenesis is a broad area of research. In this chapter, we outline the systematic dissection of vascular endothelial growth factor (VEGF)-mediated activation of endothelial nitric oxide synthase and other downstream targets that are relevant to the angiogenic response. These approaches may also be applied to most other angiogenic-factor signaling cascades.

摘要

了解细胞外生长因子如何激活促进血管生成的细胞内信号通路是一个广泛的研究领域。在本章中，我们概述了对血管内皮生长因子（VEGF）介导的内皮型一氧化氮合酶激活以及与血管生成反应相关的其他下游靶点的系统剖析。这些方法也可能适用于大多数其他血管生成因子信号级联反应。