Muller William A, Luscinskas F William
Department of Pathology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA.
Methods Enzymol. 2008;443:155-76. doi: 10.1016/S0076-6879(08)02009-0.
The inflammatory response is critical for our ability to heal wounds and fight off foreign microorganisms. Uncontrolled inflammation is also at the root of most pathologic conditions. Recruitment of leukocytes to the site of inflammation plays a defining role in the inflammatory response, and migration of leukocytes across endothelium is arguably the point of no return of the inflammatory response. Assays to study the transmigration of leukocytes have and will continue to shed light on the regulation of this vital response. Assays of transendothelial migration in vitro allow the controlled observation of this phenomenon, as well as experiments to study its regulation. In this chapter, we describe in vitro assays of transendothelial migration that have been used successfully in the authors' laboratories for decades and have proven to be reproducible, reliable, and predictive of the behavior of leukocytes and endothelial cells in models of inflammation in vivo.
炎症反应对于我们愈合伤口和抵御外来微生物的能力至关重要。不受控制的炎症也是大多数病理状况的根源。白细胞募集到炎症部位在炎症反应中起着决定性作用，而白细胞穿越内皮细胞的迁移可以说是炎症反应的不归点。研究白细胞迁移的实验已经并将继续为这一重要反应的调节提供线索。体外跨内皮迁移实验能够对这一现象进行可控观察，以及开展研究其调节机制的实验。在本章中，我们描述了体外跨内皮迁移实验，这些实验在作者的实验室中已成功使用了数十年，并且已被证明具有可重复性、可靠性，能够预测体内炎症模型中白细胞和内皮细胞的行为。
