[川芎嗪通过调节半胱天冬酶-3和神经丝蛋白表达促进脊髓修复：大鼠急性脊髓损伤模型]

[Tetramethylpyrazine accelerated spinal cord repair through regulation of caspase-3 and neurofilament protein expression: an acute spinal cord injury model in rats].

作者信息

Shen Zheng-xiang, Lü Hong-bin, Li Xiao-ming, Xu Da-qi, Hu Jian-zhong, Wang Xi-yang

机构信息

Department of Spinal Surgery, Xiangya Hospital, Central South University, Changsha 410008; Department of Spinal Surgery, First Peopleos Hospital of Yueyang, Yueyang 414000, China.

出版信息

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Aug;33(8):693-9.

PMID:18772508

Abstract

OBJECTIVE

To investigate the effect of tetramethylpyrazine on spinal cord repair through hypothesized pathways including regulation of caspase-3, neurofilament protein NF-L, NF-H, and NF-M expression.

METHODS

Allenos weight drop method was used to establish acute spinal cord injury (SCI) rat model at T10 section and was conducted in 80 adult Sprague Dawley rats that were subsequently divided into pseudo-therapy group (sodium chloride group, n=40) and tetramethylpyrazine group (treatment group, n=40). Eight normal rats were included into the pseudo-surgery normal group (n=1 for each time point). The function of spinal cord was evaluated with animal behavioral scores by measuring modified Rivilin loxotic plate degree and counting BBB score at 1, 3, 7, 14, and 21 d postoperatively. The injured spinal cord tissue samples were harvested at 1 h, 3 h, 6 h, 1 d, 3 d, 7 d, 14 d, and 21 d postoperatively (n=5 for each time point) for the preparation of continuous histological sections that were analyzed the expression of caspase-3,NF-L,NF-H, and NF-M by immunohistochemistry method.

RESULTS

At postoperative Day 7, 14, and 21, animal behavioral scores revealed higher modified Rivlin loxotic plate degree and BBB scores in the treatment group when compared with those of the control group (P<0.05). The cells in the injured spinal cord tissue expressed significantly less caspase-3 and significantly more NF (NF-L, NF-H and NF-M) in the treatment group than those in the control group at Day 3, 7, and 14 (P<0.05). There was positive correlation between modified Rivlin loxotic plate degree and NF expression, BBB score and NF expression; and negative correlations between BBB score and caspase-3 expression, caspase-3 expression and NF expression.

CONCLUSION

Tetramethylpyrazine improves spinal cord healing through regulation of caspase-3 and neurofilament protein expression.

摘要

目的

通过包括调节半胱天冬酶 - 3、神经丝蛋白NF - L、NF - H和NF - M表达等假设途径，研究川芎嗪对脊髓修复的影响。

方法

采用重物打击法在T10节段建立急性脊髓损伤（SCI）大鼠模型，选取80只成年Sprague Dawley大鼠，随后分为假治疗组（氯化钠组，n = 40）和川芎嗪组（治疗组，n = 40）。将8只正常大鼠纳入假手术正常组（每个时间点n = 1）。术后1、3、7、14和21天通过测量改良Rivlin倾斜板角度和计算BBB评分，用动物行为评分评估脊髓功能。术后1小时、3小时、6小时、1天、3天、7天、14天和21天采集损伤脊髓组织样本（每个时间点n = 5），制备连续组织学切片，采用免疫组织化学方法分析半胱天冬酶 - 3、NF - L、NF - H和NF - M的表达。

结果

术后第7、14和21天，动物行为评分显示治疗组的改良Rivlin倾斜板角度和BBB评分高于对照组（P < 0.05）。术后第3、7和14天，治疗组损伤脊髓组织中的细胞表达的半胱天冬酶 - 3明显少于对照组，而NF（NF - L、NF - H和NF - M）明显多于对照组（P < 0.05）。改良Rivlin倾斜板角度与NF表达、BBB评分与NF表达呈正相关；BBB评分与半胱天冬酶 - 3表达、半胱天冬酶 - 3表达与NF表达呈负相关。