Zhang Chun, He Xijing, Lan Binshang, Li Haopeng
Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710004, P.R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Jan;23(1):8-13.
To investigate the synergetic effect and possibility of repairing spinal cord injury (SCI) by transplantation of olfactory ensheathing cells (OECs) and chondroitinase ABC (ChABC) in adult rats.
Three adult male SD rats were used to isolated olfactory bulb and primarily cultured OECs. In the 8th or 9th day, OECs were transplanted, the concentration of cells was modulated to 1 x 10(5)/microL. Fifty-four SD rats were made the models of T8 spinal cord crush injury and divided into 4 groups. In group A (control, n = 36), injured site was not treated; in groups B, C and D (n = 6), OECs, ChABC and OECs+ChABC were injected into injured site, respectively. At 1, 2, 3, 7 and 14 days after injury, the BBB score system was used to evaluate the motion function. At 0, 1, 2, 3, 7, 14 days in group A and at 14 days in groups B, C, D after injury, the maximal transverse diameter and gross area of necrosis were evaluated on HE stained sections. The immunofluorescence double labeling staining for glial-fibrillary acidic protein (GFAP)/CS56, GFAP/growth associated protein 43 (GAP-43) and GFAP/neurofilament 160 (NF160) was carried out to evaluate the regeneration of nerve fiber.
At 14 days after injury, there were significant difference in the BBB scores between group A and groups B, C, D (P < 0.05), and between groups B, C and group D (P < 0.05), HE staining showed that the formation of cavity was observed in each group at 14 days after injury. There were significant difference in the maximal transverse diameter and gross area of necrosis between groups B, C, D and group A (P < 0.01), and between groups B, C and group D (P < 0.01). The immunofluorescence staining indicated that expression of GFAP were more intense in group A than in other groups, and the cavity of the lesion site was apparent, but it was moderate in groups B and C. The expression of GAP-43 was more intense in group D than in groups B and C. The expression of NF160 was more intense in group D.
Transplantation strategy of OECs combined with ChABC was effective in the repair of SCI in some extent.
探讨成年大鼠嗅鞘细胞（OECs）与软骨素酶ABC（ChABC）联合移植修复脊髓损伤（SCI）的协同作用及可能性。
取3只成年雄性SD大鼠分离嗅球并原代培养OECs。在第8或9天进行OECs移植，将细胞浓度调至1×10(5)/μL。将54只SD大鼠制作成T8脊髓挫伤损伤模型并分为4组。A组（对照组，n = 36），损伤部位不做处理；B、C、D组（n = 6），分别将OECs、ChABC及OECs + ChABC注入损伤部位。在损伤后1、2、3、7和14天，采用BBB评分系统评估运动功能。在损伤后A组的0、1、2、3、7、14天及B、C、D组的14天，对HE染色切片评估坏死灶的最大横径和总面积。进行胶质纤维酸性蛋白（GFAP）/CS56、GFAP/生长相关蛋白43（GAP - 43）和GFAP/神经丝160（NF160）免疫荧光双标记染色以评估神经纤维再生情况。
损伤后14天，A组与B、C、D组的BBB评分有显著差异（P < 0.05），B、C组与D组之间也有显著差异（P < 0.05）；HE染色显示损伤后14天每组均可见空洞形成。B、C、D组与A组之间坏死灶的最大横径和总面积有显著差异（P < 0.01），B、C组与D组之间也有显著差异（P < 0.01）。免疫荧光染色表明A组GFAP表达比其他组更强，损伤部位空洞明显，而B、C组为中度。D组GAP - 43表达比B、C组更强。D组NF160表达更强。
OECs联合ChABC的移植策略在一定程度上对SCI修复有效。
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