Hatch Ekaterina P, Urness Lisa D, Mansour Suzanne L
Department of Human Genetics, University of Utah, Salt Lake City, Utah, USA.
Dev Dyn. 2009 Feb;238(2):358-66. doi: 10.1002/dvdy.21681.
Fibroblast growth factors play important roles in inner ear development. Previous studies showed that mouse Fgf16 is expressed asymmetrically during the otic cup and vesicle stages of development, suggesting roles in regulating or responding to anteroposterior axial cues. Here, we studied otic Fgf16 expression throughout embryonic development and found transcripts in the developing cristae and in a few cells in the lateral wall of the cochlear duct. To determine the otic function of Fgf16 and to follow the fate of Fgf16-expressing cells, we generated an Fgf16(IRESCre) allele. We show that Fgf16 does not have a unique role in inner ear development and that the Fgf16 lineage is found throughout the three cristae, in portions of the semicircular canal ducts, and in the cochlear spiral prominence epithelial cells. This strain will be useful for gene ablations in these tissues.
成纤维细胞生长因子在内耳发育中发挥重要作用。先前的研究表明，小鼠Fgf16在发育的耳杯和囊泡阶段不对称表达，提示其在调节或响应前后轴线索中发挥作用。在此，我们研究了整个胚胎发育过程中耳内Fgf16的表达情况，发现在发育中的嵴以及耳蜗管侧壁的少数细胞中有转录本。为了确定Fgf16的耳功能并追踪表达Fgf16的细胞的命运，我们生成了一个Fgf16(IRESCre)等位基因。我们发现Fgf16在内耳发育中没有独特作用，并且Fgf16谱系存在于三个嵴、部分半规管管道以及耳蜗螺旋隆起上皮细胞中。该品系将有助于在这些组织中进行基因敲除。
