Department of Biological Structure, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98109, USA.
Dev Dyn. 2010 Mar;239(3):1019-26. doi: 10.1002/dvdy.22236.
Many studies have shown the importance of the fibroblast growth factor (FGF) family of factors in the development of the mammalian cochlea. There are four fibroblast growth factor receptors (FGFR1-4) and all four are expressed in the cochlea during development. While there are examples in the literature of expression patterns of some of the receptors at specific stages of cochlear development there has been no systematic study. We have assembled a full analysis of the patterns of receptor expression during cochlear development for all four Fgfrs using in situ hybridization. We have analyzed the expression patterns from embryonic day 13.5 through postnatal ages. We find that Fgfr1, 2, and 3 are expressed in the epithelium of the cochlear duct and Fgfr4 is limited in its expression to the mesenchyme surrounding the duct. We compare the receptor expression pattern to markers of the sensory domain (p27kip1) and the early hair cells (math1).
许多研究表明，成纤维细胞生长因子（FGF）家族在哺乳动物耳蜗发育中的重要性。有四个成纤维细胞生长因子受体（FGFR1-4），在发育过程中，这四个受体都在耳蜗中表达。尽管文献中有一些受体在耳蜗发育特定阶段表达模式的例子，但还没有进行系统的研究。我们使用原位杂交技术，对所有四个 Fgfrs 在耳蜗发育过程中的受体表达模式进行了全面分析。我们分析了从胚胎第 13.5 天到出生后各个时期的表达模式。我们发现 Fgfr1、2 和 3 都在耳蜗管的上皮细胞中表达，而 Fgfr4 的表达仅限于管周围的间质。我们将受体表达模式与感觉域标记物（p27kip1）和早期毛细胞（math1）进行了比较。
