Sawaguchi S, Iwata K, Kaiya T
Department of Ophthalmology, Niigata University School of Medicine, Japan.
Nippon Ganka Gakkai Zasshi. 1991 Apr;95(4):311-7.
The distribution and morphological characterization of the sulfated-proteoglycans in the normal human lamina cribrosa under the electron microscope after staining with cationic-dye (Cuprolinic-Blue) in critical electrolyte condition were studied. Three types of Cuprolinic-Blue positive filaments were identified associated with collagen in the lamina cribrosa according to their size, electro-density and their relation to the collagen fibrils. The thin and short Cuprolinic-Blue positive filaments (referred to as CB-1 filament in this communication) distributed over the collagen fibers in relatively regular fashion. The intermediate-sized Cuprolinic-Blue positive filaments (CB-2) were seen randomly associated with collagen fibers. The clusters of large CB-3 filaments were seen unevenly associated with loosely packed collagen fibers. The higher electron density of CB-2 and CB-3 filaments than those of CB-1 filaments were also demonstrated. Cuprolinic-Blue positive dots and blots as well as various sizes of filaments could be identified in the basal lamina surrounding the collagen beams and in the basement membrane of the blood vessels.
在临界电解质条件下用阳离子染料（铜叶绿酸蓝）染色后，在电子显微镜下研究了正常人筛板中硫酸化蛋白聚糖的分布和形态特征。根据其大小、电子密度及其与胶原纤维的关系，在筛板中鉴定出三种与胶原相关的铜叶绿酸蓝阳性细丝。细而短的铜叶绿酸蓝阳性细丝（在本通讯中称为CB-1细丝）以相对规则的方式分布在胶原纤维上。中等大小的铜叶绿酸蓝阳性细丝（CB-2）随机地与胶原纤维相关联。可见大的CB-3细丝簇与松散排列的胶原纤维不均匀地相关联。还证实了CB-2和CB-3细丝的电子密度高于CB-1细丝。在胶原束周围的基膜和血管的基底膜中可以识别出铜叶绿酸蓝阳性点和斑点以及各种大小的细丝。
