Fukuchi T, Sawaguchi S, Yue B Y, Iwata K, Hara H, Kaiya T
Department of Ophthalmology, Niigata University School of Medicine, Japan.
Exp Eye Res. 1994 Feb;58(2):231-43. doi: 10.1006/exer.1994.1012.
The distribution of the sulfated proteoglycans in the lamina cribrosa of normal monkey eyes and monkey eyes with laser-induced glaucoma were analysed by electron microscopy after cuprolinic blue dye binding. Three types of cuprolinic blue-positive filaments, CB-1, CB-2 and CB-3, were identified in the laminar beams of normal monkey eyes. CB-1 and CB-2 were both small filaments representing chondroitin and dermatan sulfate proteoglycan copolymers. The former lined up perpendicularly to the long axis of collagen fibrils, whereas the latter ran axially parallel to collagen fibrils. The large CB-3 filaments, representing chondrotin sulfate proteoglycans, were located around collagen bundles or in loose spaces within the beams. In addition, small CB-4 filaments or punctate structures representing heparan sulfate proteoglycans were found aligned on the basal laminae of blood vessels and glial cells. In the glaucomatous eyes, accumulation and enlargement of collagen-associated proteoglycan filaments were seen, accompanied by the destruction of collagenous beams. Accumulation of chondroitin sulfate proteoglycans was most evident. Prominent filamentous heparan sulfate/heparin proteoglycans were also noted in thickened astrocytic and vascular basal laminae. These may be alterations secondary to the destruction of collagen bundles. They may also represent cellular responses related to intraocular pressure elevation.
通过铜叶绿酸蓝染料结合后的电子显微镜分析，研究了正常猴眼和激光诱导性青光眼猴眼筛板中硫酸化蛋白聚糖的分布情况。在正常猴眼的板层束中鉴定出三种类型的铜叶绿酸蓝阳性细丝，即CB-1、CB-2和CB-3。CB-1和CB-2均为小细丝，代表硫酸软骨素和硫酸皮肤素蛋白聚糖共聚物。前者垂直于胶原纤维的长轴排列，而后者与胶原纤维轴向平行。代表硫酸软骨素蛋白聚糖的大的CB-3细丝位于胶原束周围或板层束内的疏松间隙中。此外，发现代表硫酸乙酰肝素蛋白聚糖的小的CB-4细丝或点状结构排列在血管和神经胶质细胞的基膜上。在青光眼眼中，可见与胶原相关的蛋白聚糖细丝的积聚和增粗，同时伴有胶原束的破坏。硫酸软骨素蛋白聚糖的积聚最为明显。在增厚的星形胶质细胞和血管基膜中也注意到显著的丝状硫酸乙酰肝素/肝素蛋白聚糖。这些可能是胶原束破坏后的继发性改变。它们也可能代表与眼压升高相关的细胞反应。
