Tena-Suck Martha Lilia, Moreno-Jiménez Sergio, Alonso Mario, Aguirre-Crux Lucinda, Sánchez Aurora
Department of Neuropathology, National Institute of Neurology and Neurosurgery, México City, México.
Ann Diagn Pathol. 2008 Oct;12(5):313-21. doi: 10.1016/j.anndiagpath.2008.02.002. Epub 2008 Jun 12.
Oligodendroglioma usually arise in adults and rarely in children. The objective of the current study was to evaluate the immunoexpression of glial fibrillary acidic protein (GFAP) and heat shock proteins (HSP70), endothelial vascular growth factor (EVGF), and endothilial vascular growth factor receptor type II (EFGF-R) expression in relation to the proliferation labeling index (proliferating cell nuclear antigen [PCNA]) and vascular density in patients with oligodendroglioma. We studied 28 cases of oligodendrogliomas--20 (71.4%) were oliodendrogliomas (grade II), and 8 (28.6%) cases were anaplastic oligodendroglioma (grade II according to World Health Organization classification). Eleven cases were male (39.28%) and 9 (32.14%) cases were female. Mitosis were found in grade II (0.35 +/- 1.14) and grade III (3.88 +/- 1.81) (P = .0001*) and pleomorphism in grade II (4.40 +/- 0.99) and grade III (9.50 +/- 9.20) (P = .028). The GFAP was positive in grade II (1.45 +/- 0.60) and grade III (2.63 +/- 0.52) (P = .000); HSP70 was immunoreactive in grade II (1.35 +/- 0.59) and grade III (2.50 +/- 0.53) (P = .001); and EVGF was immunoreactive in grade II (22.70 +/- 6.10) and grade III (36 +/- 1.63) (P = .043). The EVGF-RII was immunoreactive in grade II, 18.30 +/- 6.11 and 31.63 +/- 4.93 (P = .045). The microvascular density labeling index rates were 20.70 +/- 4.34 (grade II) and 33.38 +/- 5.29 (P = .000), and the PCNA labeling index rates were 32.95 +/- 5.89 (grade II) and 56.88 +/- 5.62 (grade III) (P = .045). We observed astrocyte differentiation in oligodendrogliomas grade III. We found a higher PGAF, HSP70, EVGF, and EFGF-R expression in relation with the PCNA and vascular density (CD34) in patients with oligodendroglioma grade III than in oligodendroglioma grade II. There was a significant relationship between mitosis, glial fibrillary acidic protein (GFAP), HSP70, EVGF, EVGF-receptor II expression, and the histologic grade and size of the tumor. For that reason, we suggest that the correlation between GFAP and HSP70 could have a relationship with the protection mechanism of the tumor itself.
少突胶质细胞瘤通常发生于成人，儿童罕见。本研究的目的是评估少突胶质细胞瘤患者中胶质纤维酸性蛋白（GFAP）、热休克蛋白（HSP70）、内皮血管生长因子（EVGF）和内皮血管生长因子II型受体（EFGF-R）的免疫表达与增殖标记指数（增殖细胞核抗原[PCNA]）和血管密度的关系。我们研究了28例少突胶质细胞瘤——20例（71.4%）为少突胶质细胞瘤（二级），8例（28.6%）为间变性少突胶质细胞瘤（根据世界卫生组织分类为三级）。11例为男性（39.28%），9例（32.14%）为女性。二级（0.35±1.14）和三级（3.88±1.81）发现有丝分裂（P = 0.0001*），二级（4.40±0.99）和三级（9.50±9.20）发现多形性（P = 0.028）。GFAP在二级（1.45±0.60）和三级（2.63±0.52）呈阳性（P = 0.000）；HSP70在二级（1.35±0.59）和三级（2.50±0.53）具有免疫反应性（P = 0.001）；EVGF在二级（22.70±6.10）和三级（36±1.63）具有免疫反应性（P = 0.043）。EVGF-RII在二级具有免疫反应性，为18.30±6.11和31.63±4.93（P = 0.045）。微血管密度标记指数率二级为20.70±4.34，三级为33.38±5.29（P = 0.000），PCNA标记指数率二级为32.95±5.89，三级为56.88±5.62（P = 0.045）。我们在三级少突胶质细胞瘤中观察到星形胶质细胞分化。我们发现三级少突胶质细胞瘤患者中PGAF、HSP70、EVGF和EFGF-R的表达与PCNA和血管密度（CD34）的相关性高于二级少突胶质细胞瘤患者。有丝分裂、胶质纤维酸性蛋白（GFAP）、HSP70、EVGF、EVGF受体II表达与肿瘤的组织学分级和大小之间存在显著关系。因此，我们认为GFAP和HSP70之间的相关性可能与肿瘤本身的保护机制有关。
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