血小板衍生生长因子的表达与人类少突胶质细胞瘤的肿瘤分级和增殖活性相关。

Platelet-derived growth factor expression correlates with tumor grade and proliferative activity in human oligodendrogliomas.

作者信息

Majumdar Kaushik, Radotra Bishan D, Vasishta Rakesh K, Pathak Ashish

机构信息

Department of Histopathology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.

出版信息

Surg Neurol. 2009 Jul;72(1):54-60. doi: 10.1016/j.surneu.2008.10.001.

DOI:10.1016/j.surneu.2008.10.001

PMID:19559929

Abstract

BACKGROUND

For the last one and a half decade, it has been found that platelet-derived growth factor (PDGF) promotes glial tumor growth through autocrine and paracrine loops, by expression of PDGFalpha receptor (PDGFRalpha) on glioma cells and PDGFbeta receptor (PDGFRbeta) on proliferating endothelial cells. However, studies on oligodendrogliomas, correlating expression of PDGF and its receptor with tumor grade and proliferative activity, through MIB-1 labeling index (LI) are relatively few as compared to astroglial counterpart.

METHODS

Formalin-fixed paraffin-embedded tissues from 55 cases of oligodendrogliomas (34 World Health Organization [WHO] grade II and 21 WHO grade III tumors) were subjected to immunohistochemistry. MIB-1 LI was calculated, and a semiquantitative scoring system for expression of PDGF and PDGFRalpha was used.

RESULTS

MIB-1 LI and PDGF expression increased with histologic grades of malignancy ("t" test, P < .001 and Mann Whitney test, U = 109, P < .001 respectively). The PDGF expression scores had a positive correlation with MIB-1 LI, irrespective of tumor grade (Pearson's correlation coefficient, r = 0.566; P < .001). However, there was no significant difference of PDGFRalpha expression between 2 grades of tumors.

CONCLUSIONS

The results of this study showed that MIB-1 LI is a rapid and cost-effective modality for predicting tumor grade in oligodendrogliomas. Immunohistochemistry for PDGF was found to be useful in differentiating various grades of oligodendroglioma, and therefore, it may be involved in tumor cell proliferation and malignant transformation. Platelet-derived growth factor receptor alpha, although expressed in oligodendroglial neoplasms, was not found to be useful in predicting tumor grade.

摘要

背景

在过去十五年间，研究发现血小板衍生生长因子（PDGF）通过自分泌和旁分泌循环促进胶质肿瘤生长，这一过程是通过胶质瘤细胞上的血小板衍生生长因子α受体（PDGFRα）以及增殖内皮细胞上的血小板衍生生长因子β受体（PDGFRβ）实现的。然而，与星形胶质细胞瘤相比，通过MIB-1标记指数（LI）研究少突胶质细胞瘤中PDGF及其受体的表达与肿瘤分级和增殖活性之间关系的研究相对较少。

方法

对55例少突胶质细胞瘤（34例世界卫生组织[WHO]二级肿瘤和21例WHO三级肿瘤）的福尔马林固定石蜡包埋组织进行免疫组织化学检测。计算MIB-1 LI，并采用半定量评分系统评估PDGF和PDGFRα的表达。

结果

MIB-1 LI和PDGF表达随组织学恶性程度分级增加而升高（分别为t检验，P < 0.001；Mann-Whitney检验，U = 109，P < 0.001）。无论肿瘤分级如何，PDGF表达评分与MIB-1 LI呈正相关（Pearson相关系数，r = 0.566；P < 0.001）。然而，两个分级的肿瘤之间PDGFRα表达无显著差异。