Kiris Ilker, Kapan Sahin, Kilbas Aynur, Yilmaz Nigar, Altuntaş Irfan, Karahan Nermin, Okutan Huseyin
Department of Cardiovascular Surgery, Suleyman Demirel University Medical School, Isparta, Turkey.
J Surg Res. 2008 Oct;149(2):206-13. doi: 10.1016/j.jss.2007.12.752. Epub 2008 Jan 10.
Renal injury induced by aortic ischemia-reperfusion (IR) is an important factor in the development of postoperative acute renal failure following abdominal aortic surgery. The purpose of this study is to examine the effect of erythropoietin on renal injury induced by aortic IR in rats.
Twenty-four Wistar-Albino rats were randomized into 3 groups (8 per group). The control group underwent laparotomy and dissection of the infrarenal abdominal aorta without occlusion. The aortic IR group underwent clamping of the infrarenal abdominal aorta for 30 min followed by 60 min of reperfusion. The aortic IR + erythropoietin group underwent the same aortic IR periods and was pretreated with 1000 U/kg subcutaneous erythropoietin 5 min before ischemia. In rat kidney specimens, tissue levels of malondialdehyde (MDA), superoxide dismutase, catalase, and glutathione peroxidase were measured. Histological evaluation of the rat kidney tissues was also done.
Aortic IR significantly increased the levels of MDA and superoxide dismutase (P < 0.05 versus control). Erythropoietin significantly decreased the levels of MDA, superoxide dismutase, and catalase (P < 0.05 versus aortic IR). Histological evaluation showed that aortic IR significantly increased (P < 0.05 versus control), whereas erythropoietin significantly decreased (P < 0.05 versus aortic IR) the focal glomerular necrosis, dilation of Bowman's capsule, degeneration of tubular epithelium, necrosis in tubular epithelium, interstitial inflammatory infiltration, and congestion of blood vessels.
The results indicate that erythropoietin has protective effects on renal injury induced by aortic IR in rats.
主动脉缺血再灌注（IR）所致肾损伤是腹主动脉手术后发生术后急性肾衰竭的重要因素。本研究旨在探讨促红细胞生成素对大鼠主动脉IR所致肾损伤的影响。
将24只Wistar白化大鼠随机分为3组（每组8只）。对照组行剖腹术并解剖肾下腹主动脉但不阻断。主动脉IR组阻断肾下腹主动脉30分钟，随后再灌注60分钟。主动脉IR + 促红细胞生成素组经历相同的主动脉IR时段，并在缺血前5分钟皮下注射1000 U/kg促红细胞生成素进行预处理。测定大鼠肾脏标本中丙二醛（MDA）、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的组织水平。还对大鼠肾脏组织进行了组织学评估。
主动脉IR显著升高了MDA和超氧化物歧化酶水平（与对照组相比，P < 0.05）。促红细胞生成素显著降低了MDA、超氧化物歧化酶和过氧化氢酶水平（与主动脉IR组相比，P < 0.05）。组织学评估显示，主动脉IR显著增加（与对照组相比，P < 0.05），而促红细胞生成素显著降低（与主动脉IR组相比）局灶性肾小球坏死、鲍曼囊扩张、肾小管上皮变性、肾小管上皮坏死、间质炎性浸润和血管充血（P < 0.05）。
结果表明促红细胞生成素对大鼠主动脉IR所致肾损伤具有保护作用。
