Buchachenko Anatoly L, Kuznetsov Dmitry A
N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow 119991, Russia.
J Am Chem Soc. 2008 Oct 1;130(39):12868-9. doi: 10.1021/ja804819k. Epub 2008 Sep 6.
The rate of ATP synthesis by creatine kinase extracted from V. xanthia venom was shown to depend on the magnetic field. The yield of ATP produced by enzymes with 24Mg2+ and 26Mg2+ ions in catalytic sites increases by 7-8% at 55 mT and then decreases at 80 mT. For enzyme with 25Mg2+ ion in a catalytic site, the ATP yield increases by 50% and 70% in the fields 55 and 80 mT, respectively. In the Earth field the rate of ATP synthesis by enzyme, in which Mg2+ ion has magnetic nucleus 25Mg, is 2.5 times higher than that by enzymes, in which Mg2+ ion has nonmagnetic, spinless nuclei 24Mg or 26Mg. Both magnetic field effect and magnetic isotope effect demonstrate that the ATP synthesis is an ion-radical process, affected by Zeeman interaction and hyperfine coupling in the intermediate ion-radical pair.
从黄斑蝰蛇毒液中提取的肌酸激酶合成ATP的速率被证明取决于磁场。催化位点含有24Mg2+和26Mg2+离子的酶产生的ATP产量在55 mT时增加7-8%，然后在80 mT时下降。对于催化位点含有25Mg2+离子的酶，在55 mT和80 mT的磁场中，ATP产量分别增加50%和70%。在地球磁场中，Mg2+离子具有磁性核25Mg的酶合成ATP的速率比Mg2+离子具有非磁性、无自旋核24Mg或26Mg的酶高2.5倍。磁场效应和磁同位素效应都表明，ATP合成是一个离子自由基过程，受中间离子自由基对中的塞曼相互作用和超精细耦合影响。
