Popov A P, Tsvetkov I L, Konichev A S
Moscow State Regional University, Moscow, Russia.
Biochemistry (Mosc). 2008 Aug;73(8):937-42. doi: 10.1134/s0006297908080129.
Deoxyribonucleases (DNases) differing in the subcellular localization and specificity towards native and denatured substrates and in products of cleavage of endogenous DNA were isolated for the first time from the hepatopancreas of freshwater snail by differential sedimentation and preparative isoelectrofocusing. It was defined found that treatment with phenol leads to activation of most of the investigated DNases, especially in lysosomes, and induces two new DNases of lysosomal origin. The possible different participation of certain DNases of the snail hepatopancreas in the regulation of DNA degradation under intoxication is discussed.
首次通过差速沉降和制备性等电聚焦从淡水蜗牛的肝胰腺中分离出了脱氧核糖核酸酶（DNases），这些酶在亚细胞定位、对天然和变性底物的特异性以及内源性DNA裂解产物方面存在差异。研究发现，用苯酚处理会导致大多数被研究的DNases激活，尤其是在溶酶体中，并诱导产生两种新的溶酶体起源的DNases。文中讨论了蜗牛肝胰腺中某些DNases在中毒情况下对DNA降解调控中可能存在的不同参与情况。
