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在厚壁菌门中鉴定编码叶酸和硫胺素结合膜蛋白的基因。

Identification of genes encoding the folate- and thiamine-binding membrane proteins in Firmicutes.

作者信息

Eudes Aymerick, Erkens Guus B, Slotboom Dirk J, Rodionov Dmitry A, Naponelli Valeria, Hanson Andrew D

机构信息

Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

出版信息

J Bacteriol. 2008 Nov;190(22):7591-4. doi: 10.1128/JB.01070-08. Epub 2008 Sep 5.

DOI:10.1128/JB.01070-08
PMID:18776013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2576666/
Abstract

Genes encoding high-affinity folate- and thiamine-binding proteins (FolT, ThiT) were identified in the Lactobacillus casei genome, expressed in Lactococcus lactis, and functionally characterized. Similar genes occur in many Firmicutes, sometimes next to folate or thiamine salvage genes. Most thiT genes are preceded by a thiamine riboswitch.

摘要

在干酪乳杆菌基因组中鉴定出编码高亲和力叶酸和硫胺素结合蛋白（FolT、ThiT）的基因，这些基因在乳酸乳球菌中表达并进行了功能表征。许多厚壁菌门细菌中都存在类似基因，有时与叶酸或硫胺素补救基因相邻。大多数thiT基因之前都有一个硫胺素核糖开关。

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本文引用的文献

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Improved methods for the preparation of [3H]folate polyglutamates: biosynthesis with Lactobacillus casei and enzymatic synthesis with Escherichia coli folylpolyglutamate synthetase.[3H]叶酸多聚谷氨酸制备方法的改进：用干酪乳杆菌进行生物合成以及用大肠杆菌叶酸多聚谷氨酸合成酶进行酶促合成。
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Biotin uptake in prokaryotes by solute transporters with an optional ATP-binding cassette-containing module.原核生物中生物素通过带有一个可选的含ATP结合盒模块的溶质转运蛋白的摄取。
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Flavin binding to the high affinity riboflavin transporter RibU.黄素与高亲和力核黄素转运蛋白RibU的结合。
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