González Félix H D, Tecles Fernando, Martínez-Subiela Silvia, Tvarijonaviciute Asta, Soler Laura, Cerón José J
Veterinary School, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
J Vet Diagn Invest. 2008 Sep;20(5):580-4. doi: 10.1177/104063870802000507.
Acute phase proteins (APPs) are important diagnostic indicators of inflammatory disturbances in animals. The objectives of the current study were to validate analytical methods for measuring haptoglobin (Hp), serum amyloid A (SAA), acid soluble glycoprotein (ASG), fibrinogen, and albumin concentrations in goats and to evaluate their response to an inflammatory stimulus in this species. Intra- and interassay coefficients of variation (CVs) were in the range 0.07-9.31% and 1.83-12.68%, respectively, for all APPs and showed good precision. All assays determined APPs in a linear manner at different sample dilutions with high correlation coefficients with the exception of fibrinogen, which was measured by the heat precipitation method. Subcutaneous injection of turpentine oil induced an increase in Hp, SAA, ASG, and fibrinogen serum concentrations and a decrease in albumin concentration.
急性期蛋白（APPs）是动物炎症紊乱的重要诊断指标。本研究的目的是验证山羊中测量触珠蛋白（Hp）、血清淀粉样蛋白A（SAA）、酸溶性糖蛋白（ASG）、纤维蛋白原和白蛋白浓度的分析方法，并评估该物种对炎症刺激的反应。所有APPs的批内和批间变异系数（CVs）分别在0.07 - 9.31%和1.83 - 12.68%范围内，显示出良好的精密度。除通过热沉淀法测量的纤维蛋白原外，所有检测方法在不同样品稀释度下均以线性方式测定APPs，且相关系数较高。皮下注射松节油可导致血清中Hp、SAA、ASG和纤维蛋白原浓度升高，白蛋白浓度降低。
