Kato Yoshio
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1, Higashi, Tsukuba, 305-8562, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):71-2. doi: 10.1093/nass/nrn036.
Methods for specific detection of RNA molecules have been widely developed in biotechnology and diagnostic field. The diagnostic target is recently expanded to non-coding RNA, including microRNAs (miRNAs). miRNAs are initially synthesized as precursor, and then processed to their physiologically active species, miRNAs. Detection of only the active mature miRNA without interference of the precursor forms is extremely important for their functional and physiological significance. We developed a novel fluorescent DNA probe to detect the mature miRNA with high specificity. Since the probe discriminates the mature miRNA from the precursor without electrophoretic separation, it should be useful for real-time detection of the mature miRNAs.
在生物技术和诊断领域，已广泛开发出用于特异性检测RNA分子的方法。最近，诊断目标已扩展到非编码RNA，包括微小RNA（miRNA）。miRNA最初以前体形式合成，然后加工成其具有生理活性的形式，即miRNA。仅检测活性成熟miRNA而不受前体形式的干扰，对于其功能和生理意义极为重要。我们开发了一种新型荧光DNA探针，用于高特异性地检测成熟miRNA。由于该探针无需电泳分离就能区分成熟miRNA和前体，因此它应该可用于实时检测成熟miRNA。
