Miyajima Yoshitaka, Ishizuka Takumi, Komiyama Makoto
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 komaba, Meguro-ku, Tokyo, 153-8904, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):125-6. doi: 10.1093/nass/nrn064.
Double-duplex invasion of pseudo-complementary PNA (pcPNA) to double-stranded DNA is promising for recognition of a specific sequence in double-stranded DNA. In order to apply this process for various purposes such as gene suppression, one base-pair change at the target site in DNA must be strictly distinguished by the pcPNA additives. In this study, mismatch-recognizing activity of double-duplex invasion was investigated under various salt conditions. It has been found that the mismatch-recognition of the invasion is strict enough to distinguish one base-pair alternation, as long as the invasion is achieved in the media of appropriate ionic strength (e.g., [NaCl] = 20 mM).
伪互补肽核酸（pcPNA）对双链DNA的双双链侵入有望用于识别双链DNA中的特定序列。为了将此过程应用于诸如基因抑制等各种目的，pcPNA添加剂必须严格区分DNA靶位点上的一个碱基对变化。在本研究中，研究了在各种盐条件下双双链侵入的错配识别活性。已经发现，只要在适当离子强度的介质（例如，[NaCl]=20 mM）中实现侵入，侵入的错配识别就足够严格，能够区分一个碱基对的交替。
