单链结合蛋白增强肽核酸链对双链DNA的侵入。
Single-strand binding protein enhances invasion of a PNA strand to double-stranded DNA.
作者信息
Ishizuka Takumi, Komiyama Makoto
机构信息
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, Japan.
出版信息
Nucleic Acids Symp Ser (Oxf). 2008(52):141-2. doi: 10.1093/nass/nrn072.
PMID:18776293
Abstract
We show that, in the presence of single-strand binding protein (SSB), one strand of peptide nucleic acid (PNA) efficiently invades double-stranded DNA (dsDNA) in sequence-specific manner, as shown in Figure 1. In the absence of SSB, this kind of strand invasion never occurs. This significant enhancement of invasion can be applied to in vivo applications.
摘要
如图1所示，我们发现，在单链结合蛋白（SSB）存在的情况下，肽核酸（PNA）的一条链能够以序列特异性方式有效侵入双链DNA（dsDNA）。在没有SSB的情况下，这种链侵入从未发生。这种显著增强的侵入作用可应用于体内应用。
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