Nakano Shun, Hasegawa Tetsuya, Fukuda Masatora, Fujieda Nobutaka, Tainaka Kazuki, Morii Takashi
Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):199-200. doi: 10.1093/nass/nrn101.
We describe here a ribonucleopeptide (RNP) receptor targeting a tetra-amino-acid motif containing phosphotyrosine, GpYSR. GpYSR-binding RNP receptors were obtained from an RNA-based RNP library by in vitro selection. These receptors have a higher affinity than those of previously obtained pY-binding RNP receptors. One of these RNP receptors exhibited unique specificity to the target GpYSR peptide over other tetra-amino-acid peptides derived from the tyrosine-phosphorylation sites of native proteins. The GpYSR-binding RNP receptor discriminated not only the phosphorylated tyrosine residue, but also its surrounding three amino acid residues. Thus, RNP receptors could target a defined pY-containing amino-acid sequence by expanding the recognition surface within the ligand-binding pocket of RNP.
我们在此描述一种核糖核蛋白（RNP）受体，其靶向包含磷酸酪氨酸的四氨基酸基序GpYSR。通过体外筛选从基于RNA的RNP文库中获得了结合GpYSR的RNP受体。这些受体比先前获得的结合磷酸化酪氨酸（pY）的RNP受体具有更高的亲和力。其中一种RNP受体对靶标GpYSR肽表现出独特的特异性，优于源自天然蛋白质酪氨酸磷酸化位点的其他四氨基酸肽。结合GpYSR的RNP受体不仅能区分磷酸化的酪氨酸残基，还能区分其周围的三个氨基酸残基。因此，RNP受体可以通过扩大RNP配体结合口袋内的识别表面来靶向特定的含pY氨基酸序列。
