含2,6 - 二氨基嘌呤和2 - 硫代胸苷锁核酸碱基的DNA双链体的稳定性和错配识别

Stability and mismatch discrimination of DNA duplexes containing 2,6-diaminopurine and 2-thiothymidine locked nucleic acid bases.

作者信息

Hughesman Curtis B, Turner Robin F B, Haynes Charles

机构信息

Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):245-6. doi: 10.1093/nass/nrn124.

DOI:10.1093/nass/nrn124

PMID:18776345

Abstract

Hybridization thermodynamics measured by differential scanning calorimetry (DSC) and UV spectroscopy (UVM) are reported for 8- and 14-mer oligonucleotides containing two new LNA bases: 2,6 diaminopurine (D) and 2-thiothymidine (2sT). Oligonucleotides containing D or 2sT bases are shown to have enhanced stability and improved discrimination for several of the possible mismatched base pairs.

摘要

通过差示扫描量热法（DSC）和紫外光谱法（UVM）测量了含有两种新型锁核酸（LNA）碱基：2,6-二氨基嘌呤（D）和2-硫代胸苷（2sT）的8聚体和14聚体寡核苷酸的杂交热力学。结果表明，含有D或2sT碱基的寡核苷酸对几种可能的错配碱基对具有更高的稳定性和更好的区分能力。