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基于表面等离子体共振成像技术在寡核苷酸阵列上进行的分析,以检测修复蛋白对DNA损伤的识别和切除。

SPR-imaging based assays on an oligonucleotide-array to analyze DNA lesions recognition and excision by repair proteins.

作者信息

Corne C, Fiche J-B, Cunin V, Buhot A, Fuchs J, Calemczuk R, Favier A, Livache T, Gasparutto D

机构信息

Laboratoire Lésions des Acides Nucléiques, SCIB/UMR E3 CEA-UJF, INAC, CEA-Grenoble, F-38054 Grenoble Cedex 9, France.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):249-50. doi: 10.1093/nass/nrn126.

DOI:10.1093/nass/nrn126
PMID:18776347
Abstract

An original oligonucleotide-array, coupled with SPR-imaging detection, has been developed to study biological interactions between DNA base lesions and DNA repair enzymes. This bioanalytical tool constitutes an efficient screening platform to quantify DNA repair activities and to search for new DNA repair inhibitors.

摘要

一种结合表面等离子体共振成像检测的原始寡核苷酸阵列已被开发出来,用于研究DNA碱基损伤与DNA修复酶之间的生物相互作用。这种生物分析工具构成了一个高效的筛选平台,可用于量化DNA修复活性并寻找新的DNA修复抑制剂。

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