用于含7,8-二氢-8-氧代鸟嘌呤的双链DNA的2',4'-双环核酸探针的设计与化学合成
Design and chemical synthesis of a 2',4'-BNA probe for 7,8-dihydro-8-oxoguanine-containing double stranded DNA.
作者信息
Miyoshi Tetsuya, Kodama Tetsuya, Obika Satoshi, Imanishi Takeshi
机构信息
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
出版信息
Nucleic Acids Symp Ser (Oxf). 2008(52):305-6. doi: 10.1093/nass/nrn154.
7,8-Dihydro-8-oxoguanine (8-OxoG), caused by DNA oxidation, is considered to be a marker of oxidative stress, and also to be a cause of genomic diversity in organism because of its mutagenic potential. Here, we designed and synthesized a 2',4'-BNA probe for 8-OxoG-containing double stranded DNA (dsDNA). The designed probe showed an 8-OxoG:C base pair selective triplex-forming ability.
由DNA氧化产生的7,8-二氢-8-氧代鸟嘌呤（8-氧代鸟嘌呤，8-OxoG）被认为是氧化应激的标志物，并且由于其诱变潜力，也是生物体基因组多样性的一个原因。在此，我们设计并合成了一种用于含8-氧代鸟嘌呤的双链DNA（dsDNA）的2',4'-双环核酸（BNA）探针。所设计的探针显示出对8-氧代鸟嘌呤:C碱基对具有选择性的三链体形成能力。
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