Kuwahara Masayasu, Minezaki Satoshi, Nagashima Jun-ichi, Ozaki Hiroaki, Sawai Hiroaki
Graduate School of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):453-4. doi: 10.1093/nass/nrn230.
We synthesized two types of modified DNA templates in which the phosphodiester linkage was replaced with an amide-type linker [-CH(2)C=ONH-] or an amine-type linker [-CH(2)CH(2)NH-]. Primer extension reactions were performed using these modified DNA templates to investigate the effect of backbone modification on polymerase reactions. Our results indicated that the polymerase reaction was affected much more by the insertion of the cationic flexible amine-type linker than by insertion of the neutral rigid amide-type linker.
我们合成了两种类型的修饰DNA模板，其中磷酸二酯键被酰胺型接头[-CH(2)C=ONH-]或胺型接头[-CH(2)CH(2)NH-]取代。使用这些修饰的DNA模板进行引物延伸反应，以研究主链修饰对聚合酶反应的影响。我们的结果表明，与中性刚性酰胺型接头的插入相比，阳离子柔性胺型接头的插入对聚合酶反应的影响要大得多。
