Kimoto Michiko, Kawai Rie, Mitsui Tsuneo, Yokoyama Shigeyuki, Hirao Ichiro
Systems and Structural Biology Center, Yokohama Institute, RIKEN, Yokohama, Kanagawa, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):457-8. doi: 10.1093/nass/nrn232.
Unnatural base pairs, compatible with PCR amplification, could potentially increase the versatility of nucleic acids. We recently reported an unnatural base pair, between 7-(2-thienyl)-imidazo[4,5-b]pyridine (denoted by Ds) and 2-nitropyrrole (denoted by Pn), which specifically and efficiently functions in PCR. Toward the efficient incorporation of extra, functional components into DNA fragments, we examined the influence of the sequences around the unnatural base pair and the dependence of the substrate concentrations on the selectivity and efficiency of replication by DNA polymerase.
与聚合酶链式反应（PCR）扩增兼容的非天然碱基对，有可能增加核酸的多功能性。我们最近报道了一种非天然碱基对，它存在于7-（2-噻吩基）-咪唑并[4,5-b]吡啶（记为Ds）和2-硝基吡咯（记为Pn）之间，在PCR中具有特异性且高效的功能。为了将额外的功能性成分有效掺入DNA片段，我们研究了非天然碱基对周围序列的影响以及底物浓度对DNA聚合酶复制选择性和效率的依赖性。
