Maie Kenji, Nakamura Mitsunobu, Yamana Kazushige
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Himeji, Hyogo 671-2201, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):705-6. doi: 10.1093/nass/nrn356.
We studied the RNA oligomers having pyrenylmethyl substituents at the 2'-O-sugar residues. It has been shown that the pyrene-modified RNAs can form duplexes with complementary RNA sequences without loss of thermal stability. Absorption, fluorescence, and circular dichroism spectra revealed that the multiply incorporated pyrenes are projected toward the outside of RNA duplexes and assembled in helical aromatic arrays along the minor grooves of the RNA duplexes. The helical pyrene arrays exhibited remarkably strong excimer fluorescence. In the present work, we carried out the fluorescence quenching of the excimer derived from the pyrene-arrays assembled on RNA duplexes in order to evaluate the mobility of the exciton along the pyrene array.
我们研究了在2'-O-糖残基处带有芘甲基取代基的RNA低聚物。已表明芘修饰的RNA可与互补RNA序列形成双链体，且不会损失热稳定性。吸收光谱、荧光光谱和圆二色光谱显示，多个掺入的芘朝向RNA双链体的外侧突出，并沿着RNA双链体的小沟以螺旋芳香族阵列形式组装。螺旋芘阵列表现出非常强的激基缔合物荧光。在本工作中，我们对组装在RNA双链体上的芘阵列产生的激基缔合物进行了荧光猝灭，以评估激子沿芘阵列的迁移率。
