Hirano Masanori, Shimada Naohiko, Kano Arihiro, Kidoaki Satoru, Maruyama Atsushi
Institute for Materials Chemistry and Engineerng, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-8581, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):715-6. doi: 10.1093/nass/nrn361.
We have reported that poly(L-lysine)-graft-dextran (PLL-g-Dex) accelerates DNA hybridization and increases stability of a double stranded DNA (dsDNA). Furthermore, PLL-g-Dex was found to stimulate the DNA strand exchange reaction between dsDNA and its complementary single stranded DNA (ssDNA). In order to better understand these phenomena, we evaluate PLL-g-Dex/DNA interaction by the single molecular observation and intermolecular force measurement at single molecular level. We have been able to observe PLL-g-Dex/DNA complex at single molecular level. PLL-g-Dex was found to increases the dissociation force of a dsDNA.
我们曾报道聚（L-赖氨酸）接枝葡聚糖（PLL-g-Dex）能加速DNA杂交并提高双链DNA（dsDNA）的稳定性。此外，还发现PLL-g-Dex能刺激dsDNA与其互补单链DNA（ssDNA）之间的DNA链交换反应。为了更好地理解这些现象，我们通过单分子观察和单分子水平的分子间力测量来评估PLL-g-Dex/DNA相互作用。我们已经能够在单分子水平观察到PLL-g-Dex/DNA复合物。发现PLL-g-Dex能增加dsDNA的解离力。
