Barnard J W, Barman S A, Adkins W K, Longenecker G L, Taylor A E
Department of Physiology, College of Medicine, University of South Alabama, Mobile 36688.
Am J Physiol. 1991 Aug;261(2 Pt 2):H479-86. doi: 10.1152/ajpheart.1991.261.2.H479.
Effects of endothelin-1 (10(-8) M) on the pulmonary vascular resistance-compliance profile were examined in isolated blood-perfused rabbit, dog, and rat lungs using occlusion techniques. Capillary permeability was assessed by filtration coefficient (Kfc). Cyclooxygenase products were assessed by radioimmunoassay. In rabbit lungs, endothelin-1 increased all resistances except large vein; ibuprofen reversed the constriction. Endothelin-1 decreased total vascular compliance (CT), which was reversed by ibuprofen. Cyclooxygenase products were unchanged by endothelin-1 or endothelin-1 plus ibuprofen. In dog lungs, large vein resistance increased after endothelin-1; ibuprofen increased large arterial resistance. Endothelin-1 decreased CT and middle compartment compliance, and endothelin-1 plus ibuprofen decreased large vessel compliance (CLV). Ibuprofen reversed the endothelin-1 increase in plasma 6-oxo-prostaglandin F1 alpha. In rat lungs, ibuprofen reversed the endothelin-1 increase in small arterial resistance. Endothelin-1 decreased CLV, and endothelin-1 plus ibuprofen returned the compliance to baseline values. Ibuprofen potentiated the endothelin-1 increase in plasma prostanoids. Endothelin-1 plus indomethacin increased vascular resistance and blocked prostaglandin production. Kfc was increased only in rat lung after endothelin-1 plus ibuprofen. In summary, endothelin-1 increased pulmonary vascular resistance, which was attenuated by prostacyclin in dogs and rats. In rabbits, the resistance increase was reversed by ibuprofen.
采用闭塞技术，在离体血液灌注的兔、犬和大鼠肺中研究了内皮素-1（10⁻⁸ M）对肺血管阻力-顺应性曲线的影响。通过滤过系数（Kfc）评估毛细血管通透性。通过放射免疫分析法评估环氧化酶产物。在兔肺中，内皮素-1增加了除大静脉外的所有阻力；布洛芬可逆转这种收缩。内皮素-1降低了总血管顺应性（CT），布洛芬可使其逆转。内皮素-1或内皮素-1加布洛芬对环氧化酶产物无影响。在犬肺中，内皮素-1后大静脉阻力增加；布洛芬增加了大动脉阻力。内皮素-1降低了CT和中间隔顺应性，内皮素-1加布洛芬降低了大血管顺应性（CLV）。布洛芬逆转了内皮素-1引起的血浆6-氧代-前列腺素F1α增加。在大鼠肺中，布洛芬逆转了内皮素-1引起的小动脉阻力增加。内皮素-1降低了CLV，内皮素-1加布洛芬使顺应性恢复到基线值。布洛芬增强了内皮素-1引起的血浆前列腺素增加。内皮素-1加吲哚美辛增加了血管阻力并阻断了前列腺素的产生。仅在内皮素-1加布洛芬处理后的大鼠肺中，Kfc增加。总之，内皮素-1增加了肺血管阻力，在犬和大鼠中，前列环素可减弱这种作用。在兔中，布洛芬可逆转阻力增加。
