Shvets Elena, Elazar Zvulun
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel.
Autophagy. 2008 Nov;4(8):1054-6. doi: 10.4161/auto.6823. Epub 2008 Nov 20.
LC3 is a widely used marker of autophagosomes in mammalian cells. However, in addition to its autophagosomal localization, GFP-LC3 is often found associated with protein aggregates that are formed in an autophagy-independent manner. In addition, LC3 directly interacts with p62/SQSTM1 (hereafter named p62), a common constituent of protein aggregates. In our recent report, we mapped the regions in LC3 involved in its binding to p62 and showed that this binding is essential for the incorporation of p62 into autophagosomes. Here we demonstrate that the autophagy-unrelated association of GFP-LC3 with protein aggregates is dependent on its interaction with p62.
LC3是哺乳动物细胞中自噬体广泛使用的标志物。然而，除了其自噬体定位外，GFP-LC3经常被发现与以非自噬方式形成的蛋白质聚集体相关。此外，LC3直接与p62/SQSTM1（以下简称p62）相互作用，p62是蛋白质聚集体的常见成分。在我们最近的报告中，我们绘制了LC3中参与其与p62结合的区域，并表明这种结合对于p62纳入自噬体至关重要。在这里，我们证明GFP-LC3与蛋白质聚集体的非自噬相关关联取决于其与p62的相互作用。
