Kuliczkowska Justyna, Filus Alicja, Trzmiel Anna, Tworowska Urszula, Demissie Marek, Jedrzejuk Diana, Milewicz Andrzej
Department of Endocrinology, Diabetology and Isotope Therapy, Medical University of Wroclaw, Poland.
Endokrynol Pol. 2008 Jul-Aug;59(4):312-5.
The aim of this study was to examine the association of Pro12Ala PPARgamma2 polymorphism with anthropometric and biochemical parameters defining the risk for the development of metabolic syndrome in a healthy population of men.
The study group consisted of 176 healthy men, aged 25-65 years (average 54.16 years). Polymorphisms of the PPAR-g gene (Pro12Ala, Ala12Ala, Pro12Pro) were explored using the PCR-RFLP method. Plasma glucose, insulin, total cholesterol, LDL, HDL and TG were measured using commercially available kits.
The genotypic distribution of the Pro12Ala polymorphism was as follows: Pro/Ala 69.8% (n = 123), Ala/Ala 28.4% (n = 50) and Pro/Pro 1.8% (n = 3). The Pro12Ala and Ala12Ala subjects did not differ in any of the measured variables. The non-obese (BMI < 30 kg/m(2), n = 117) and obese subpopulations (BMI > 30 kg/m(2), n = 56) did not significantly differ in the distribution of the genotypes. In the nonobese subpopulation, the homozygous Ala12 carriers (n = 38, 32.4%) had higher systolic blood pressure, plasma triglycerides, insulin levels and HOMA-IR.
We conclude that despite the high frequency of the Ala allele at the PPAR-gamma2 gene in our population of Polish men, the Ala12 allele does not appear to improve insulin sensitivity or have an influence on the occurrence of obesity. It remains to be explained by larger studies if this polymorphism carries any risk of the development of metabolic abnormalities in non-obese men.
本研究旨在探讨Pro12Ala PPARγ2基因多态性与男性健康人群中定义代谢综合征发生风险的人体测量和生化参数之间的关联。
研究组由176名年龄在25 - 65岁（平均54.16岁）的健康男性组成。采用PCR-RFLP方法检测PPAR-γ基因的多态性（Pro12Ala、Ala12Ala、Pro12Pro）。使用市售试剂盒测量血浆葡萄糖、胰岛素、总胆固醇、低密度脂蛋白、高密度脂蛋白和甘油三酯。
Pro12Ala多态性的基因型分布如下：Pro/Ala 69.8%（n = 123），Ala/Ala 28.4%（n = 50），Pro/Pro 1.8%（n = 3）。Pro12Ala和Ala12Ala受试者在任何测量变量上均无差异。非肥胖（BMI < 30 kg/m²，n = 117）和肥胖亚组（BMI > 30 kg/m²，n = 56）在基因型分布上无显著差异。在非肥胖亚组中，纯合Ala12携带者（n = 38，32.4%）的收缩压、血浆甘油三酯、胰岛素水平和HOMA-IR较高。
我们得出结论，尽管在我们的波兰男性人群中PPAR-γ2基因的Ala等位基因频率较高，但Ala12等位基因似乎并未改善胰岛素敏感性或对肥胖的发生有影响。这种多态性是否会给非肥胖男性带来代谢异常发展的风险，还有待更大规模的研究来解释。
