Mazumder S, Bose S, Bandopadhyay A, Alam S, Mukherjee M
Indian Institute of Chemical Biology, Kolkata, India.
Lett Appl Microbiol. 2008 Oct;47(4):355-60. doi: 10.1111/j.1472-765X.2008.02418.x.
To achieve high laccase production from Pleurotus ostreatus in a bench top bioreactor and to utilize the enzyme for determination of the total antioxidant concentration (TAC) of human plasma.
Laccase production by P. ostreatus studied in a benchtop bioreactor was as high as, 8740 U ml(-1) in presence of copper sulfate. The enzyme was used to replace metmyoglobin and hydrogen peroxide for the estimation of TAC in human plasma. The trolox equivalent antioxidant concentrations determined by the laccase-based method and metmyoglobin method ranged from 1.63 +/- 0.011 to 1.80 +/- 0.006 mmol l(-1) and from 1.41 +/- 0.004 to 1.51 +/- 0.008 mmol l(-1) plasma, respectively.
Pleurotus ostreatus produced high amount of extracellular laccase in a benchtop bioreactor. The enzyme can be used to assay TAC of blood plasma without the interference encountered with the hydrogen peroxide and metmyoglobin mediated assay method.
Laccase production by P. ostreatus obtained in this study was the highest among all reported laccase producing white-rot fungi. Moreover, an accurate laccase-based assay method was developed for detection of TAC in human plasma.
在台式生物反应器中实现平菇高产漆酶，并利用该酶测定人血浆中的总抗氧化剂浓度（TAC）。
在台式生物反应器中研究平菇产生漆酶的情况，在硫酸铜存在下，漆酶产量高达8740 U ml⁻¹。该酶被用于替代高铁肌红蛋白和过氧化氢来估算人血浆中的TAC。用基于漆酶的方法和高铁肌红蛋白方法测定的Trolox当量抗氧化剂浓度，在血浆中分别为1.63±0.011至1.80±0.006 mmol l⁻¹和1.41±0.004至1.51±0.008 mmol l⁻¹。
平菇在台式生物反应器中产生了大量胞外漆酶。该酶可用于测定血浆中的TAC，而不会受到过氧化氢和高铁肌红蛋白介导的测定方法所遇到的干扰。
本研究中平菇产生漆酶的量在所有已报道的产漆酶白腐真菌中是最高的。此外，还开发了一种准确的基于漆酶的测定方法来检测人血浆中的TAC。
