Moldestad Olve, Karlsen Pernille, Molden Sturla, Storm Johan F
Centre for Molecular Biology and Neuroscience, Department of Physiology, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Domus Medica, Sognsvannsveien 9, PB1103 Blindern, 0317 Oslo, Norway.
J Neurosci Methods. 2009 Jan 30;176(2):57-62. doi: 10.1016/j.jneumeth.2008.08.015. Epub 2008 Aug 19.
Urethane anesthesia is frequently used for acute experiments on small rodents in physiology and neuroscience. Severe respiratory distress is a common side-effect of urethane anesthesia in many strains of mice. Associated complications interfere with completion of experiments, and as a consequence more animals must be sacrificed. During experiments with stereotaxic brain surgery, we found that intubation by means of tracheotomy is an efficient way to maintain patent airways in these animals. Artificial ventilation of the animals is not required. In this paper we describe a simple, fast and reliable method for intubation of mice in experiments that involve a stereotaxic instrument. The method proved considerably easier to learn and apply than conventional intubation through the oral route. The incidence of breathing problems decreased from 77% in untreated mice to 9% in those that underwent tracheotomy. In addition, the success rate for our acute electrophysiological experiments increased from 24 to 77%. We conclude that tracheotomy reduces the number of sacrificed animals, and saves time and labor.
氨基甲酸乙酯麻醉常用于生理学和神经科学领域对小型啮齿动物进行的急性实验。严重的呼吸窘迫是许多品系小鼠使用氨基甲酸乙酯麻醉时常见的副作用。相关并发症会干扰实验的完成，结果是必须牺牲更多的动物。在进行立体定向脑部手术的实验过程中，我们发现通过气管切开术进行插管是维持这些动物气道通畅的有效方法。无需对动物进行人工通气。在本文中，我们描述了一种在涉及立体定向仪器的实验中对小鼠进行插管的简单、快速且可靠的方法。事实证明，该方法比通过口腔途径进行的传统插管更容易学习和应用。呼吸问题的发生率从未经治疗的小鼠中的77%降至接受气管切开术的小鼠中的9%。此外，我们急性电生理实验的成功率从24%提高到了77%。我们得出结论，气管切开术减少了牺牲动物的数量，并节省了时间和劳动力。
