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[沙门氏菌菌株中的氨基糖苷类失活酶及其编码基因]

[Aminoglycoside-inactivating enzymes in strains of Salmonella and genes encoding them].

作者信息

Vakulenko S B, Kolganov A N, Krotova L A

出版信息

Antibiot Khimioter. 1991 Mar;36(3):32-4.

PMID:1877874
Abstract

Aminoglycoside resistance patterns of 56 strains isolated from man, cattle and environment were determined. 34 out of 42 gentamicin-resistant strains were shown to produce AAC(3)-II and 7 strains produced ANT(2"). All the 48 kanamycin resistant strains produced APH(3')-I. Spot hybridization of the 42 gentamicin resistant strains with the inner fragment of the aacC2 gene revealed positive signals for all the strains. Hybridization of the 48 kanamycin-resistant strains with the aphA1 gene probe provided positive results in all the strains. The AAC(3)-IV encoding gene was not detected by DNA-DNA hybridization in the strains studied.

摘要

测定了从人、牛和环境中分离出的56株菌株的氨基糖苷类耐药模式。42株庆大霉素耐药菌株中有34株显示产生AAC(3)-II,7株产生ANT(2")。48株卡那霉素耐药菌株均产生APH(3')-I。42株庆大霉素耐药菌株与aacC2基因内部片段的斑点杂交显示所有菌株均呈阳性信号。48株卡那霉素耐药菌株与aphA1基因探针杂交在所有菌株中均得到阳性结果。在所研究的菌株中,通过DNA-DNA杂交未检测到编码AAC(3)-IV的基因。

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