Kolganov A N, Vakulenko S B
Antibiot Khimioter. 1992 Nov;37(11):10-4.
The patterns of aminoglycoside inactivating enzymes were determined by AGRP in 31 clinical isolated of Serratia marcescens. The results were compared with the data on identification of the aminoglycoside resistance genes by the specific DNA probes. It was shown that all the isolates of Serratia marcescens contained the AAC(6')-Ic gene which was not expressed in some isolates. The other detected aminoglycoside inactivating enzymes were the following: AAC(3)-V in 17 isolates, ANT(2'') in 7 isolates, AAC(3)-I in 4 isolates and APH(3')-I in 13 isolates. Reliability of the methods of AGRP and DNA-DNA hybridization was estimated in the assay of the aminoglycoside resistant clinical strains of Serratia marcescens.
农业研究计划（AGRP）测定了31株临床分离的粘质沙雷氏菌中氨基糖苷类灭活酶的模式。将结果与通过特异性DNA探针鉴定氨基糖苷类抗性基因的数据进行了比较。结果表明，所有粘质沙雷氏菌分离株均含有AAC(6')-Ic基因，其中一些分离株未表达该基因。检测到的其他氨基糖苷类灭活酶如下：17株中有AAC(3)-V，7株中有ANT(2'' )，4株中有AAC(3)-I，13株中有APH(3')-I。在对粘质沙雷氏菌的氨基糖苷类耐药临床菌株的检测中评估了AGRP和DNA-DNA杂交方法的可靠性。
