Adami P, Berrez J M, Latruffe N
Laboratoire de Biologie Moléculaire et Cellulaire, Université de Bourgogne, Dijon, France.
Biochem Int. 1991 Feb;23(3):429-37.
Primary biliary cirrhosis is an evolutive and chronic human liver disease characterized by presence of antimitochondrial autoantibodies in the serum. We present the biochemical definition of these autoantigens and show that purified pyruvate dehydrogenase complex contains three of the major mitochondrial antigens of M2 type i.e. the E2 subunit (dihydrolipoamide acetyl transferase), the X subunit and the E1 alpha subunit (pyruvate dehydrogenase), by immunoblotting experiments and inhibition of enzyme activity with several types of serums.
原发性胆汁性肝硬化是一种渐进性慢性人类肝脏疾病，其特征是血清中存在抗线粒体自身抗体。我们给出了这些自身抗原的生化定义，并通过免疫印迹实验以及用几种血清抑制酶活性，证明纯化的丙酮酸脱氢酶复合物包含三种主要的M2型线粒体抗原，即E2亚基（二氢硫辛酰胺乙酰转移酶）、X亚基和E1α亚基（丙酮酸脱氢酶）。
