糖尿病Goto-Kakizaki大鼠Descemet膜中长间距胶原的变化及其抗糖尿病药物的抑制作用。
Change in long-spacing collagen in Descemet's membrane of diabetic Goto-Kakizaki rats and its suppression by antidiabetic agents.
作者信息
Akimoto Yoshihiro, Sawada Hajime, Ohara-Imaizumi Mica, Nagamatsu Shinya, Kawakami Hayato
机构信息
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan.
出版信息
Exp Diabetes Res. 2008;2008:818341. doi: 10.1155/2008/818341.
We examined changes in the ultrastructure and localization of major extracellular matrix components, including 5 types of collagen (type I, III, IV, VI, and VIII), laminin, fibronectin, and heparan sulfate proteoglycan in Descemet's membrane of the cornea of diabetic GK rats. In the cornea of diabetic GK rats, more long-spacing collagen fibrils were observed in Descemet's membrane than in the membrane of the nondiabetic Wistar rats. Both GK and Wistar rats showed an age-dependent increase in the density of the long-spacing collagen. Immunoelectron microscopy showed that type VIII collagen was localized in the internodal region of the long-spacing collagen, which was not labelled by any of the other antibodies used. The antidiabetic agents nateglinide and glibenclamide significantly suppressed the formation of the long-spacing collagen in the diabetic rats. Long-spacing collagen would thus be a useful indicator for studying diabetic changes in the cornea and the effect of antidiabetic agents.
我们研究了糖尿病GK大鼠角膜后弹力层中主要细胞外基质成分的超微结构变化和定位，这些成分包括5种胶原蛋白（I型、III型、IV型、VI型和VIII型）、层粘连蛋白、纤连蛋白和硫酸乙酰肝素蛋白聚糖。在糖尿病GK大鼠的角膜中，后弹力层中观察到的长间距胶原纤维比非糖尿病Wistar大鼠的角膜后弹力层更多。GK大鼠和Wistar大鼠的长间距胶原密度均呈现年龄依赖性增加。免疫电子显微镜显示，VIII型胶原定位于长间距胶原的节间区域，而其他所使用的抗体均未标记该区域。抗糖尿病药物那格列奈和格列本脲可显著抑制糖尿病大鼠长间距胶原的形成。因此，长间距胶原将成为研究角膜糖尿病变化及抗糖尿病药物作用的有用指标。
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