Ohnaka K, Takayanagi R, Yamauchi T, Umeda F, Nawata H
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Biochem Int. 1991 Feb;23(3):499-506.
The incubation of big endothelin-1 (big ET-1), big ET-2 or big ET-3 with cultured bovine endothelial cells (ECs) resulted in their conversions to mature endothelins (ETs). These conversions apparently exhibited Michaelis-Menten kinetics as a function of each big ET isopeptide. The conversions of big ETs were abolished by phosphoramidon. These results indicate that vascular endothelium can convert exogenous big ET-1 to mature ET-1 through a phosphoramidon-sensitive metalloprotease, and that this enzyme has also high affinities for big ET-2 and big ET-3.
将大内皮素-1（big ET-1）、大内皮素-2或大内皮素-3与培养的牛内皮细胞（ECs）一起温育，结果它们转化为成熟的内皮素（ETs）。这些转化显然呈现出作为每种大ET同工肽函数的米氏动力学。大ETs的转化被磷酰胺素所抑制。这些结果表明，血管内皮可通过一种对磷酰胺素敏感的金属蛋白酶将外源性大ET-1转化为成熟的ET-1，并且该酶对大ET-2和大ET-3也具有高亲和力。
