Lei Yan-jun, Gao Chen, Jiang Hui-ying, Han Jun, Chen Jian-ming, Shi Qi, Zhou Wei, Yuan Yu-kang, Dong Xiao-ping
School of Medicine, Xian Jiao-Tong University, Xian 710061, China.
Bing Du Xue Bao. 2008 Jul;24(4):268-71.
Common warts are close associated with HPVs infection. In this study, we amplified and sequenced the LCR fragment and E2 gene of HPV-2 that infected the patient of extensive common wart with cutaneous horns, and we constructed the recombinant CAT-reporter plasmids pBLCAT-LCR containing HPV-2 prototype or variant LCR and mammalian expression plasmids pcDNA3. 1-E2 containing prototype or variant E2 ORF individually. The promoter activities of HPV-2 variant and the transcriptional repression activities of the mutated E2 protein were evaluated by transient transfection into HeLa cells. The results showed that there were several mutations in LCR and E2 gene of HPV-2 variant. Compared with the prototype, the viral early promoter activity of variant was significantly increased uder the control of LCR. Compared with the wild type E2 protein, the transcriptional repression activities of the mutated E2 protein was abolished partially. We speculate herein that increased promoter activities and decreased repression effect of the mutated E2 protein are linked, at least partially, with the clinical phenotypes of the uncommon huge common wart.
寻常疣与HPV感染密切相关。在本研究中，我们对感染了患有皮肤角的广泛寻常疣患者的HPV - 2的LCR片段和E2基因进行了扩增和测序，并分别构建了含有HPV - 2原型或变异LCR的重组CAT报告质粒pBLCAT - LCR以及含有原型或变异E2 ORF的哺乳动物表达质粒pcDNA3.1 - E2。通过瞬时转染HeLa细胞评估HPV - 2变异体的启动子活性和突变E2蛋白的转录抑制活性。结果显示，HPV - 2变异体的LCR和E2基因存在多个突变。与原型相比，变异体在LCR控制下的病毒早期启动子活性显著增加。与野生型E2蛋白相比，突变E2蛋白的转录抑制活性部分丧失。我们在此推测，突变E2蛋白启动子活性增加和抑制作用降低至少部分与罕见巨大寻常疣的临床表型有关。
